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Custom MHC Tetramers— Fast, Easy, and Cost-effective

*Please scroll down to the bottom of the page for the final deliverable.

Project Overview

Located in San Diego, BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research.

The objective of this video is to increase sales of the Flex-T product line from the BioLegend website.

Storyboard

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Do you need a reagent

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that identifies antigen-specific T-cells

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and you’re interested in recognizing a specific peptide antigen?

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Sounds like an MHC tetramer,

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but the conventional method for making tetramers with a specific peptide is a complex and labor-intensive process,

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and most commercial tetramers that are preloaded with peptide are expensive

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and limited to a small set of predefined antigens.

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This is why BioLegend offers the Flex-T technology.

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With Flex-T technology, loading your peptide-of-interest onto an MHC monomer is fast and efficient,

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and, once tetramerized,

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the custom MHC tetramer delivers highly reproducible results.

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Loading a peptide is an easy three-step process:

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Step 1: Mix your peptide-of-interest with Flex-T monomers.

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The Flex-T monomers come pre-loaded with a UV light-labile peptide.

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Step 2: Expose your sample to UV light.

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The UV light degrades the pre-loaded peptide,

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enabling binding of your peptide of interest to the MHC monomer.

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Step 3: Mix monomers with fluorophore-streptavidin to create tetramers.

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Once formed, the tetramers are ready to use.

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Available in a range of MHC alleles,

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Flex-T is a powerful, stable, and cost-effective method for studying antigen-specific T-cells for basic research, screening, and more.

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Try Flex-T today, order at biolegend.com/order-flex-t.

Video Final Deliverable

 

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