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Project Overview
Located in San Diego, BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research.
The objective of this video is to increase sales of the Flex-T product line from the BioLegend website.
Storyboard

Do you need a reagent

that identifies antigen-specific T-cells

and you’re interested in recognizing a specific peptide antigen?

Sounds like an MHC tetramer,

but the conventional method for making tetramers with a specific peptide is a complex and labor-intensive process,

and most commercial tetramers that are preloaded with peptide are expensive

and limited to a small set of predefined antigens.

This is why BioLegend offers the Flex-T technology.

With Flex-T technology, loading your peptide-of-interest onto an MHC monomer is fast and efficient,

and, once tetramerized,

the custom MHC tetramer delivers highly reproducible results.

Loading a peptide is an easy three-step process:

Step 1: Mix your peptide-of-interest with Flex-T monomers.

The Flex-T monomers come pre-loaded with a UV light-labile peptide.

Step 2: Expose your sample to UV light.

The UV light degrades the pre-loaded peptide,

enabling binding of your peptide of interest to the MHC monomer.

Step 3: Mix monomers with fluorophore-streptavidin to create tetramers.

Once formed, the tetramers are ready to use.

Available in a range of MHC alleles,

Flex-T is a powerful, stable, and cost-effective method for studying antigen-specific T-cells for basic research, screening, and more.

Try Flex-T today, order at biolegend.com/order-flex-t.